TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: TG86]

Specifications

Product Data
Clone Name	TG86
Applications	FC, IF, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Western Blotting (Not tested) Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 1-2 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 1-2 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer, Melanoma.
Reactivity	Human, Rabbit, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	A 10-amino acid synthetic peptide (aa34-43) from human TGFα.
Specificity	This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Cellular Localization: Cytoplasmic and secreted.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~6 kDa
Gene Name	transforming growth factor alpha
Database Link	Entrez Gene 7039 Human UniProt ID P01135
Synonyms	Transforming growth factor alpha, ETGF
Reference Data

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