14 3 3 gamma (YWHAG) Mouse Monoclonal Antibody [Clone ID: AT4B9]

Specifications

Product Data
Clone Name	AT4B9
Applications	ELISA, IF, WB
Recommend Dilution	ELISA. WB: 1/1000 (using lysates of HeLa (40µg) and mouse brain (40 µg), resolved by SDS-PAGE and transferred to PVDF membrane) ICC/IF: 1/100 (using HeLa cell line).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli.
Specificity	Recognizes Human 14-3-3 gamma (YWHAG). Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
Database Link	Entrez Gene 7532 Human UniProt ID P61981
Background	The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma, Epsilon, Delta, Zeta, Tau and Eta that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Synonyms	YWHAG, KCIP-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis

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