ATP7A (42-61, Cytopl. Dom.) Mouse Monoclonal Antibody [Clone ID: S60-4]

Specifications

Product Data
Clone Name	S60-4
Applications	IHC, IP, WB
Recommend Dilution	Immunohistochemistry: free floating sections, fixed in formaldehyde. Immunoprecipitation. Western Blot: 1 μg/ml of this antibody was sufficient for detection of ATP7A / Copper-transporting ATPase1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti mouse IgG-HRP as the secondary antibody
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein amino acids 42-61 (cytoplasmic, C-terminus) of human ATP7A / Copper- transporting ATPase 1
Specificity	This antibody detects ATP7A (aa42-61); ~180kDa.
Formulation	PBS pH 7.2, 50% Glycerol State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium azide
Concentration	lot specific
Purification	Protein G chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	ATPase copper transporting alpha
Database Link	Entrez Gene 538 Human UniProt ID Q04656
Background	The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage.
Synonyms	Copper pump 1, MC1, MNK
Reference Data

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