SIGLEC5 / SIGLEC14 Mouse Monoclonal Antibody [Clone ID: 1A5]

Specifications

Product Data
Clone Name	1A5
Applications	ELISA, FC, IF, IP
Recommend Dilution	Flow cytometry: 1/25-1/200, use 10µl of this working dilution to label 10e6 cells in 100µl. Immunofluorescence. Immunoprecipitation. ELISA.
Reactivity	Chimpanzee, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	SIGLEC5-Fc protein, consisting of the full-length extracellular region of human SIGLEC5, fused with the Fc region of human IgG1
Specificity	This antibody recognises human SIGLEC5 / CD170. Clone 1A5 antibody is one of several SIGLEC5 antibodies which also recognises human SIGLEC14 (Angata, T. et al., 2006).
Formulation	PBS containing 0.09% Sodium azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Siglec-5 shares an almost identical sequence with Siglec-14 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5, also known as CD170, is a sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), Siglec-5 mediates sialic-acid dependent binding to cells, and is as well acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule and predominantly expressed in hematopoietic tissues, which have been shown to associate with the activating adapter protein DAP12.
Synonyms	Q08ET2, O15389, CD170
Reference Data

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