ATF 4 (ATF4) (25-327) Mouse Monoclonal Antibody [Clone ID: S360-A-24]

Specifications

Product Data
Clone Name	S360-A-24
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/1000; 1 μg/ml was sufficient for detection of ATF4 in 20 μg of rat brain lysate by colorimetric immunoblot using HRP conjugated secondary antibody. Immunocytochemistry. Immunohistochemistry: Free floating sections, fixed in formaldehyde.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein amino acids 25-327 (nearly full-length, all but first 24 amino acids) of human ATF4; <50% identity with ATF5.
Specificity	This antibody detects 60 kDa protein. Does not cross-react with ATF5.
Formulation	PBS pH 7.4, 50% Glycerol, 0,09% Sodium azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	activating transcription factor 4
Database Link	Entrez Gene 468 Human UniProt ID P18848
Background	Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
Synonyms	CREB2, CREB-2, TXREB, Activating transcription factor 4, ATF-4
Reference Data

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