Collagen II (COL2A1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: 1/1000-1/4000.
Reactivity	Bovine
Host	Goat
Clonality	Polyclonal
Immunogen	Bovine type II collagen
Specificity	This antibody is specific for Bovine collagen type II and demonstrates less than 10% cross reactivity with bovine collagen types I, III, IV, V and VI. This antibody has not been tested for reactivity with other ECM proteins e.g. laminin and fibronectin. This antibody may cross-react with collagen II from other species.
Formulation	Borate buffered saline State: Purified State: Liquid Ig fraction Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	collagen type II alpha 1 chain
Database Link	Entrez Gene 1280 Human UniProt ID P02458
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Synonyms	COL2A1, Alpha-1 type II collagen
Reference Data
Protein Pathways	ECM-receptor interaction, Focal adhesion

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