Follistatin (FST) Rabbit Polyclonal Antibody
CAT#: AP01108PU-S
Follistatin (FST) rabbit polyclonal antibody, Aff - Purified
Size: 100 ug
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CNY 3,910.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | ELISA: Indirect: To detect Follistatin by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant Follistatin. Sandwich: To detect hFollistatin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human Follistatin as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98 %) recombinant human Follistatin |
Specificity | This antibody detects Follistatin. |
Formulation | PBS, pH 7.2 State: Aff - Purified State: Sterile filtered, lyophilized Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml. |
Purification | Immunoaffinity purified |
Conjugation | Unconjugated |
Storage Condition | Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing. |
Gene Name | follistatin |
Database Link | |
Background | Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone. There are three different isoforms, generated by alternative splicing. |
Synonyms | FST, FS, Activin-binding protein |
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