BMP7 Rabbit Polyclonal Antibody

CAT#: AP01113PU-N

BMP7 rabbit polyclonal antibody, Purified

Size: 50 ug 100 ug

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, FN, WB
Recommend Dilution Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP7 (100 ng/ml), a concentration of 0.40-0.60 µg/ml of this BMP7 antibody is required.
Sandwich ELISA:
To detect Human BMP7 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml is required. The antigen affinity purified BMP7 antibody, in conjunction with Biotinylated antibody anti-Human BMP7 antibody (AP01113BT-N or AP01113BT-S) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BMP-7.
Western Blot:
To detect Humnan BMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Hunan BMP-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen CHO cell derived recombinant highly pure (>98%) Human BMP-7.
Specificity Detects BMP7.
Formulation PBS, pH 7.2 without preservatives.
State: Purified
State: Lyophilized (Sterile filtered) purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography using an immobilized Human BMP7 matrix.
Conjugation Unconjugated
Storage Condition Store the lyophilized antibody at -20°C.
Following reconstitution it is stable for two weeks at 2-8°C.
Frozen aliquots are stable for 6 months when stored at -20 °C.
Avoid repeated freezing and thawing.
Gene Name bone morphogenetic protein 7
Background Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.
Synonyms BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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