Ccl4 Rabbit Polyclonal Antibody

CAT#: AP01132BT-N

Ccl4 rabbit polyclonal antibody, Biotin

Size: 25 ug 50 ug

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution ELISA: Direct: To detect mMIP-1β by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1β.
Sandwich: To detect mMIP-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Murine MIP-1β as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1β.
Western Blot: To detect mMIP-1β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (> 98 %) recombinant murine MIP-1β
Specificity This antibody detects Macrophage Inflammatory Protein-1 beta.
Formulation PBS, pH 7.2
Label: Biotin
State: Sterile filtered lyophilized Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
Purification Affinity chromatography
Conjugation Biotin
Storage Condition Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at
2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
Gene Name chemokine (C-C motif) ligand 4
Background MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
Synonyms CCL-4, MIP-1-beta, LAG1, MIP1B, SCYA4, Small-inducible cytokine A4, PAT744, Protein H400, SIS-gamma, HC21
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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