IGFBP5 Rabbit Polyclonal Antibody
CAT#: AP01139BT-S
IGFBP5 rabbit polyclonal antibody, Biotin
Size: 50 ug
Conjugation: Unconjugated
Need it in bulk or conjugated?
Get a free quote
CNY 3,910.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | ELISA: Direct: To detect hIGF-BP5 (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5. Sandwich: To detect hIGF-BP5 (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human IGF-BP5 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5. Western blot: To detect hIGF-BP5 this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98 %) recombinant human IGF-BP5 |
Specificity | This antibody detetcs Insulin-like Growth Factor Binding Protein 5. |
Formulation | PBS, pH 7.2 Label: Biotin State: Sterile filtered lyophilized Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml. |
Purification | Affinity chromatography |
Conjugation | Biotin |
Storage Condition | Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing. |
Gene Name | insulin like growth factor binding protein 5 |
Database Link | |
Background | IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. |
Synonyms | IGF-binding protein 5, IGFBP-5, IBP5, IBP-5 |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |