Galectin 3 (LGALS3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA Direct: To detect hGalectin-3 (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. In conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-3. Sandwich: To detect Human Galectin-3 (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human Galectin-3 (AP01146PU-N or AP01146PU-S) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-3. Western blot To detect hGalectin-3 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Galectin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) recombinant human Galectin-3
Specificity	This antibody detects Galectin-3.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	lectin, galactoside binding soluble 3
Database Link	Entrez Gene 3958 Human UniProt ID P17931
Background	Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 is normally expressed in epithelia of a variety of tissues, including colon and endometrium, and in various inflammatory cells, including macrophages. Galectin-3 can function intracellularly, in controlling cell cycle and preventing T-cell apoptosis, and also extracellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Expression of Galectin-3 is affected by neoplastic transformation, being up-regulated in certain types of lymphomas, and in thyroid and hepatic carcinomas. Conversely, it is down-regulated in other cancers, such as colon, breast, ovarian, and uterine.
Synonyms	Mac-2, Lgals3, GAL3, GALBP, CBP35, L-31
Note	Centrifuge vial prior to opening!
Reference Data

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