CCN2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human CTGF (5.0 µg/ml) a concentration of 6.0-9.0 µg/ml of this antibody is required. Sandwich ELISA: To detect Human CTGF (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This affinity purified antibody, in conjunction with Biotinylated antibody Human CTGF (Cat.-No AP01150BT) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTGF. Western blot: To detect Human CTGF this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 0.125-0.250 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human CTGF (Cat.-No PA527)
Specificity	This antibody detects Human Connective Tissue Growth Factor (CTGF). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Immunoaffinity Chromatography employing immobilized Human CTGF matrix.
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	connective tissue growth factor
Database Link	Entrez Gene 1490 Human UniProt ID P29279
Background	CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.
Synonyms	CCN2, HCS24, IGFBP8, Connective tissue growth factor, IGF-binding protein 8,IBP8
Note	Centrifuge vial prior to opening.
Reference Data

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