MIA2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Indirect ELISA: To detect hMIA-2 (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Sandwich ELISA: To detect hMIA-2 (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human MIA-2 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Western blot: To detect hMIA-2 this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98 %) E.coli derived 11.5 kDa recombinant human MIA-2
Specificity	This antibody detects MIA-2.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	CTAGE family member 5
Database Link	Entrez Gene 4253 Human UniProt ID Q96PC5
Background	MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.
Synonyms	Melanoma inhibitory activity protein 2
Reference Data

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