CYP7B1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of CYP7B1 protein. (region surrounding Gln127)
Formulation	PBS pH~7.2 with 0.05% Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction (>95% pure by SDS-PAGE)
Concentration	1.0 mg/ml
Purification	Affinity-Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 58 kDa
Gene Name	cytochrome P450 family 7 subfamily B member 1
Database Link	Entrez Gene 9420 Human UniProt ID O75881
Background	P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-α-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25-and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.
Synonyms	Cytochrome P450 7B1
Reference Data
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Primary bile acid biosynthesis

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