p21 (CDKN1A) pThr145 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of p21Cip1 p53 protein when phosphorylated at Thr145
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~21.0 kDa
Gene Name	cyclin-dependent kinase inhibitor 1A
Database Link	Entrez Gene 1026 Human UniProt ID P38936
Background	The p53-inducible gene product, p21(WAF1/CIP1), plays a critical role in regulating the rate of tumor incidence. p21 is a kinase inhibitor that was originally identified as a cyclin-dependent kinase and PCNA-binding protein ble to inhibit Cdk catalytic activity and as a gene whose expression was induced by the tumor suppressor protein p53. p21 contributes to the regulation of cell division, mediation of negative growth signals, differentiation and senescence, modulation of the apoptotic response and activaion of certain cyclin-Cdks in response to mitogenic signals. Serine 146 is a phosphorylation site within the carboxy terminal regulatory domain of p21 and when modified in vivo influences p21-PCNA interactions.
Synonyms	CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1
Reference Data

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