STAT5A pTyr694 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of STAT5A pTyr694 protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 91 kDa
Gene Name	signal transducer and activator of transcription 5A
Database Link	Entrez Gene 6776 Human UniProt ID P42229
Background	Stat5 (signal transducers and activators of transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5α and Stat5β in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5α and Stat5β on Ser 726 and Ser 731, respectively. Stat5β is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5α. Both the acid-rich region and the COOH terminus of IL-2Rβ can independently mediate IL-2-induced Stat5α/β serine phosphorylation, suggesting that Stat5α/β serine phosphorylation occurs at a postreceptor level. Stat5α is phosphorylated on Tyr 694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response. Ser 779 is constitutively phosphorylated in the mammary gland, and Ser 725 phosphorylation influences prolactin-stimulated in vitro DNA binding activity.
Synonyms	STAT-5, STAT-5A, Mammary gland factor, MGF
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.