Progesterone Receptor (PGR) pSer190 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from Human Progesterone Receptor around the phosphorylation site of Serine 190 (G-L-Sp-P-A).
Specificity	This antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at Serine 190.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% Glycerol Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	99 kDa
Gene Name	progesterone receptor
Database Link	Entrez Gene 5241 Human UniProt ID P06401
Background	Progesteron receptors (PRs) are nuclear hormone receptors of the NR3C class, which also inculdes mineralocorticoid, glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-kappaB, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary fland and receptor expression is induced by estrogen. Well established functions of progesterone receptores include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition, progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory center to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and druing pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22.
Synonyms	PR, PGR, NR3C3
Reference Data

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