Her2 (ERBB2) pTyr1248 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from Human HER2 around the phosphorylation site of Tyrosine 1248 (P-E-Yp-L-G).
Specificity	This antibody detects endogenous levels of HER2 only when phosphorylated at Tyrosine1248.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl State: Aff - Purified State: Liquid purified Ig fraction Preservative: no BSA/AMINES, and no glycerol.
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	185 kDa (Predicted)
Gene Name	erb-b2 receptor tyrosine kinase 2
Database Link	Entrez Gene 2064 Human UniProt ID P04626
Background	ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
Synonyms	HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
Reference Data

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