MAPT / TAU pSer404 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Incubate Membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide around phosphorylation site of Serine 404 (D-T-Sp-P-R) derived from Human Tau.
Specificity	AP02404PU antibody detects endogenous levels of Tau only when phosphorylated at Serine 404.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% Glycerol Preservative: 0.02% Sodium Azide
Concentration	1.0 mg/ml
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	48, 62, 78 KDa
Gene Name	microtubule associated protein tau
Database Link	Entrez Gene 4137 Human UniProt ID P10636
Background	Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.
Synonyms	MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau
Reference Data

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