PKR (EIF2AK2) pThr451 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry: 1:50~1:100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 451 (K-G-TP-L-R).
Specificity	PKR antibody detects endogenous levels of PKR only when phosphorylated at threonine 451.
Formulation	PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG
Concentration	lot specific
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	eukaryotic translation initiation factor 2 alpha kinase 2
Database Link	Entrez Gene 5610 Human UniProt ID P19525
Background	PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.
Synonyms	eIF-2A protein kinase 2, PRKR
Reference Data

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