IKB beta (NFKBIB) pSer23 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry: 1:50~1:100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human IκB-β around the phosphorylation site of serine 23 (L-G-SP-L-G ).
Specificity	IκB-β antibody detects endogenous levels of total IκB-β protein.
Formulation	PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG
Concentration	lot specific
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	NFKB inhibitor beta
Database Link	Entrez Gene 4793 Human UniProt ID Q15653
Background	Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting.
Synonyms	I-kappa-B-beta, TRIP9, IkB-B, IkB-beta, IkappaBbeta, TR-interacting protein 9
Reference Data

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