Ephrin B2 (EFNB2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide Sequence around amino acids 328~332 (N-I-Y-Y-K) derived from Human Ephrin-B2.
Specificity	This antibody AP02708PU detects endogenous levels of total Ephrin-B2 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	ephrin B2
Database Link	Entrez Gene 1948 Human UniProt ID P52799
Background	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.
Synonyms	EFNB2, EPLG5, HTKL, LERK5, HTK-L, LERK-5, HTK ligand
Note	Molecular Weight: 37 kDa
Reference Data

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