BAD pSer136/99 Rabbit Polyclonal Antibody

CAT#: AP02799PU-N

BAD pSer136/99 rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 2,250.00


货期*
2周

规格
    • 100 ul

Product images

Specifications

Product Data
Applications IHC, WB
Recommend Dilution Immunohistochemistry on paraffin sections: 1:50 - 1:100.
Western blot: see ref. 1.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthesized phosphopeptide derived from mouse BAD around the phosphorylation site of serine136
Specificity BAD (phospho-Ser136) antibody detects endogenous levels of BAD only when phosphorylated at Serine 136.
Formulation Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State: Aff - Purified
State: Liquid Ig fraction
Concentration lot specific
Purification Affinity chromatography using epitope-specific phosphopeptide
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Gene Name BCL2 associated agonist of cell death
Background Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Synonyms BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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