BAD pSer136/99 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on paraffin sections: 1:50 - 1:100. Western blot: see ref. 1.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthesized phosphopeptide derived from mouse BAD around the phosphorylation site of serine136
Specificity	BAD (phospho-Ser136) antibody detects endogenous levels of BAD only when phosphorylated at Serine 136.
Formulation	Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography using epitope-specific phosphopeptide
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Gene Name	BCL2 associated agonist of cell death
Database Link	Entrez Gene 572 Human UniProt ID Q92934
Background	Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Synonyms	BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8
Reference Data

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