Acetylated Lysine Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IP, WB
Recommend Dilution	ELISA. Western blot: A 1/250 dilution of this HRP- conjugated antibody AP03035HR-N was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis (Ref.6). Immunofluorescence. Immunoprecipitation (Ref.7).
Host	Rabbit
Clonality	Polyclonal
Immunogen	Acetylated KLH
Specificity	This antibody detects proteins containing Acetylated Lysine residues in SDS-PAGE immunoblots (Multi-species).
Formulation	PBS Label: HRP State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% Glycerol Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	HRP
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
Synonyms	AcK, acetyl Lysine, acetyl-Lysine
Reference Data

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