Methylated Lysine Rabbit Polyclonal Antibody
CAT#: AP03036HR-N
Methylated Lysine rabbit polyclonal antibody, HRP, Aff - Purified
Conjugation: Biotin
Need it in bulk or conjugated?
Get a free quote
CNY 6,776.00
货期*
4周
规格
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | ELISA. Western blot: 1/2000-1/5000 (4). 0.2-0.5 μg/ml of this HRP conjugated antibody was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt. |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Methylated KLH |
Specificity | This antibody detects proteins containing methylated Lysine residues in SDS-PAGE immunoblots (Multi-species). |
Formulation | PBS Label: HRP State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% Glycerol Preservative: 0.091% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography |
Conjugation | HRP |
Storage Condition | Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Centrifuge vial before opening. |
Background | Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6). |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...