Chk1 (CHEK1) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IP, WB
Recommend Dilution	Western Blot: 1-10 µg/ml. Immunoprecipitation.
Reactivity	Human, Monkey, Mouse, Rat
Host	Sheep
Clonality	Polyclonal
Immunogen	A GST fusion protein corresponding to amino acid sequence 1-474 with having a molecular weight of 54 kD.
Specificity	This antibody recognizes CHK1 kinase.
Formulation	Phosphate buffered saline with 0.08% Sodium Azide as preservative. State: Purified State: Liquid (0.2µm sterile filtered) purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	checkpoint kinase 1
Database Link	Entrez Gene 1111 Human UniProt ID O14757
Background	One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.
Synonyms	CHEK1, CHEK-1
Reference Data

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