VAV2 (DPH Region) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IP, WB
Recommend Dilution	Western Blot: 1/5,000. Immunoprecipitation (Empirical).
Reactivity	Human
Host	Sheep
Clonality	Polyclonal
Immunogen	A his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a Molecular Weight of 100kD
Specificity	This antibody reacts to Vav-2 DPH.
Formulation	PBS with 0.08% Sodium Azide as preservative State: Purified State: Liquid (0.2 µm sterile filtered) purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulfate Precipitation
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	vav guanine nucleotide exchange factor 2
Database Link	Entrez Gene 7410 Human UniProt ID P52735
Background	Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetiic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokenesis frequently are uncoupled.
Synonyms	VAV-2
Reference Data

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