Farnesyl Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF
Recommend Dilution	ELISA: Detects farnesyl motif derived from isoprenylated C-A-A-X sequence. Also cross-reacts with geranylgeranyl motif. Detects KLH as well. Immunofluorescence
Host	Rabbit
Clonality	Polyclonal
Immunogen	Antibody developed using Farnesyl cysteine conjugated to KLH.
Specificity	This antibody recognizes Farnesyl.
Formulation	Phosphate buffered saline with 0.08% Sodium Azide State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Ammonium Sulfate Precipitation
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Protein isoprenylation is a post-translational modification that affects about 0.5% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A 1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.
Reference Data

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