SPARC Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 0.5-1 µg/ml. Positive Control: Mouse heart lysate. Immunohistochemistry.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from the Human SPARC protein
Specificity	This antibody detects Human SPARC. May cross react with SPARCL1.
Formulation	Phosphate buffered saline with 0.08% Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Antigen Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon arrival freeze at -20°C to -70°C. Avoid repeated freezing and thawing.
Gene Name	secreted protein acidic and cysteine rich
Database Link	Entrez Gene 20692 MouseEntrez Gene 6678 Human UniProt ID P09486
Background	SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3' UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).
Synonyms	ON, Basement-membrane protein 40, BM-40
Reference Data
Protein Families	Druggable Genome

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