CERT1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot (5-10 µg/ml). Positive control: ovary.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from human CERTL protein
Specificity	This antibody detects CERTL.
Formulation	Phosphate buffered saline with 0.08% sodium azide State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C. Avoid repeated freezing and thawing.
Gene Name	collagen type IV alpha 3 binding protein
Database Link	Entrez Gene 10087 Human UniProt ID Q9Y5P4
Background	CERT mediates the ATP-dependent ER-to-Golgi transfer of ceramide in a non-vesicular manner. The biosynthesis of lipids involves steps that occur in different intracellular compartments. The movement of lipids within these compartments is important in lipid-mediated signalling. Human CERT is identical to a splice variant of human Goodpasture antigen-binding protein (GPBP26). CERT contains a phosphoinositide-binding pleckstrin-homology (PH) domain (which targets CERT to the Golgi by binding phosphatidylinositol-4-phosphate (PtdIns4P)), a middle region, and a putative lipid-transfer-catalysing domain called START. CERT and CERTL can specifically extract ceramide from phospholipid bilayers in a START-domain-dependent manner. CERT interacts with ER membranes and specifically extracts ceramide. CERT catalyses both the specific extraction of ceramide from donor vesicles and its transfer to acceptor vesicles. CERT can associate with the Golgi in a PtdIns4P dependent manner.
Synonyms	CERT, STARD11, Collagen type IV alpha-3-binding protein, Ceramide transfer protein, hCERT, Goodpasture antigen-binding protein, GPBP, StAR-related lipid transfer protein 11, START domain-containing protein 11, StARD11
Reference Data

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