PDGF beta (PDGFB) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 21-70 of Human PDGF-B.
Specificity	This antibody detects endogenous levels of PDGFB protein. (region surrounding Leu48)
Formulation	Phosphate buffered saline (PBS), pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze thaw cycles!
Predicted Protein Size	~ 27 kDa
Gene Name	platelet derived growth factor subunit B
Database Link	Entrez Gene 5155 Human UniProt ID P01127
Background	PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.
Synonyms	Platelet-derived growth factor subunit B, PDGF beta, PDGF B, PDGF-B, Platelet-derived growth factor beta, PDGF subunit B, Platelet-derived growth factor B chain, PDGF2, PDGF-2, c-Sis, SIS, Becaplermin
Reference Data

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