TGF beta 3 (TGFB3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 261-310 of Human TGF β3.
Specificity	This antibody detects endogenous levels of pro-TGF-beta-3 (47 kDa) and cleaved-TGF-beta-3 (13 kDa) protein. (region surrounding Gly292)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~50 kDa
Gene Name	transforming growth factor beta 3
Database Link	Entrez Gene 7043 Human UniProt ID P10600
Background	Transforming Growth Factor beta 3 (TGF beta 3) is a member of the TGF beta family of growth factors. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi-modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. A high level of TGF beta 3 in normal adult heart, lung, and brain suggests a possible role for this factor in the regulation of a variety of normal physiological functions. The expression of TGF beta 3 in different locations and at specific stages of embryogenesis suggests its involvement in the regulation of development. Similar to the other isoforms of TGF beta, the upregulated activation of TGF beta 3 might also be involved in a variety of pathological conditions. TGF beta 3 has been detected in human, porcine, and avian sources and is expressed in cells of mesenchymal origin, suggesting a different role for this protein than for TGF beta 1 or TGF beta 2. TGF beta 3 is less prevalent in natural expression than either TGF beta 1 or TGF beta 2, but is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord and in several human carcinoma cells.
Synonyms	Transforming growth factor beta-3
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.