GRP94 (HSP90B1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/5000 - 1/25000. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml. Western Blot: 1/500 - 1/2500.
Reactivity	Chicken, Drosophila, Human, Monkey, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide.
Specificity	Amino acids 289-300 of human Hsp90 protein.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preserevative. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein 90kDa beta family member 1
Database Link	Entrez Gene 22027 MouseEntrez Gene 362862 RatEntrez Gene 699568 MonkeyEntrez Gene 7184 Human UniProt ID P14625
Background	Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3.
Synonyms	Tumor rejection antigen 1, GRP94
Reference Data
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer

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