HSF1 pSer307 Rabbit Polyclonal Antibody

CAT#: AP08015PU-N

HSF1 pSer307 rabbit polyclonal antibody, Aff - Purified



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CNY 2,250.00


货期*
2周

规格
    • 100 ul

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Specifications

Product Data
Applications IF, WB
Recommend Dilution Western blot (1/500-1/1000).
Immunofluorescence (1/100-1/200).
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).
Specificity This antibody HSF1 (pSer307) detects endogenous levels of HSF1 only when phosphorylated at Serine 307.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name heat shock transcription factor 1
Background All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction.

Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.

HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.
Synonyms HSTF1, HSF-1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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