Vimentin (VIM) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry: 1/50-1/100 using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do not require antigen retrieval. Positive Control: Sarcomas, melanomas, Hela cells.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	14 amino acid peptide from C-terminal of Human Vimentin.
Specificity	This antibody reacts with Human Vimentin. Other species not tested. Cellular Localization: Cytoplasmic.
Formulation	10mM PO4, 200mM NaCl, pH 7.4 State: Purified State: Liquid purified Total Ig fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is 57 kDa intermediate filament proteins in mesenchymal cells. It is attached to the nucleus, endoplasmic reticulum and mitochondria either laterally or terminally. Vimentin is important in the differential diagnosis of undifferentiated neoplasm.
Synonyms	VIM
Reference Data

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