Human Kappa Light Chain Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes). Recommended positive control: Human tonsil.
Reactivity	Human, Monkey
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified human Kappa light chain from human Bence Jones proteins.
Specificity	This antibody is useful in identification of leukemias, plasmasacytomas and certain non-Hodgkin's lymphomas. In normal human serum lambda chains are present 1.6-15.2 mg/ml. In multiple myeloma or B-cell malignancies these levels are increased. Higher amounts are also present in Bence Jones patient's urine. Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 containing 0.05% Sodium Azide as preservative and 1% BSA as stabilizer. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30; most of them are bound to heavy chains of immunoglobulin.
Reference Data

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