Grin1 (C2' Splice Variant) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000 (unpublished observations).
Reactivity	Bovine, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide from the NR1 subunit, C2' splice variant insert of rat NMDA Receptor.
Specificity	This antibody recognizes the ~120k NR1 subunit of the NMDA receptor containing the C2' splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2' insert.
Formulation	5 mM Ammonium Bicarbonate. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in 50 µl PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	glutamate ionotropic receptor NMDA type subunit 1
Database Link	Entrez Gene 24408 Rat UniProt ID P35439
Background	The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity (Grosshans et al., 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
Synonyms	NMDAR1,GRIN1
Note	Purification Method: Prepared from Rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
Reference Data

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