Heparanase 1 (HPSE) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 1/500.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human 50kD-8kD Heparanase Heterodimer recombinant protein.
Specificity	Recognizes the 65kD precursor as well as the 50kD and 8kD subunits of Human or Mouse Heparanase.
Formulation	PBS, pH 7.2 containing 0.001% Thimerosal as preservative State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heparanase
Database Link	Entrez Gene 10855 Human UniProt ID Q9Y251
Background	Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.
Synonyms	HEP, HPA, HPA1, HPR1, HPSE1, HSE1, Heparanase-1, Endo-Glucoronidase
Note	Predicted Molecular Weight: 62 kDa.
Reference Data

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