Collagen IV (COL4A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml. Immunoprecipitation. Western Blot.
Reactivity	Bovine, Human, Mammalian
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen Type IV from Human and Bovine placenta.
Specificity	This antibody reacts with most Mammalian Type IV Collagens and has negligible cross-reactivity with type I, II, III, V, or VI Collagens.
Formulation	0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to opening. This product is stable at 2-8°C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	collagen type IV alpha 1 chain
Database Link	Entrez Gene 1282 Human UniProt ID P02462
Background	Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Synonyms	COL4A1
Reference Data
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

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