Glucose Oxidase Sheep Polyclonal Antibody

CAT#: AP09102PU-S

Glucose Oxidase sheep polyclonal antibody, Purified

Conjugation: Unconjugated HRP



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CNY 8,025.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution ELISA: 1/5,500-1/25,000.
Western blot: 1/500-1/3,000.
Immunoprecipitation.
This antibody has been assayed against 1.0 µg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using peroxidase conjugated affinity purified anti-sheep IgG (rabbit) and (ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested.
Reactivity Human
Host Sheep
Clonality Polyclonal
Immunogen Glucose Oxidase from Aspergillus niger
Specificity This antibody reacts to Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.  Immunoelectrophoresis give a single precipitin arc against anti-sheep serum as well as purified and partially purified Glucose Oxidase.
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
State: Purified
State: Lyophilized IgG fraction
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid.
Synonyms Glucose oxyhydrase, Beta-D-glucose:oxygen 1-oxido-reductase, gox
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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