Biotin Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	Immunofluorescence. Flow Cytometry.
Host	Goat
Clonality	Polyclonal
Immunogen	Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
Specificity	This antibody reacts to Fluorescein conjugated IgG fraction of Biotin.
Formulation	0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: DTAF State: Lyophilized IgG fraction Label: Diaminotriazynlaminofluorescein Absorption emission: 495 nm / 528 nm Molar radio: 1.7 moles DTAF per mole of Goat IgG
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	DTAF
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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