CCL2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/4500. Western Blot: 1 µg/ml.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein
Specificity	This antibody reacts to MCP1 / CCL2.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide State: Aff - Purified State: Liquid (sterile filtered)
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 281043 Bovine UniProt ID P28291
Background	CCL2 is a small cytokine belonging to the CC chemokine family that recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing a signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a total molecular weight of approximately 8.8 kDa. The cell surface receptors that bind CCL2 are CCR2 and CCR4. CCL2 is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). In human osteoclasts, CCL2 and RANTES (regulated on activation normal T cell expressed and secreted) may act as an autocrine loop in human osteoclast differentiation. CCL2 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines.
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Reference Data

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