HA Epitope Tag (YPYDVPDYA) Rabbit Polyclonal Antibody

CAT#: AP09230PU-N

HA Epitope Tag (YPYDVPDYA) rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin HRP



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CNY 5,371.00


货期*
4周

规格
    • 100 ug

Cited in 1 publication.

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Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope.
The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors.
This antibody has been tested by ELISA and Western blotting against both the immunizing peptide and HA containing recombinant proteins.
This antibody is likely functional for Immunoprecipitation, Immunocytochemistry, and other Immunodetection techniques.
Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Recommended Dilutions
ELISA: 1/10,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,000.
Host Rabbit
Clonality Polyclonal
Immunogen Whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from Hemagglutinin Influenza conjugated to KLH using maleimide.
A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Specificity This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays.
This anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag.
To date, it has reacted with all HA-tagged proteins tested. In Western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
State: Aff - Purified
State: Liquid (sterile filtered) Ig fraction
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Concentration lot specific
Purification Affinity Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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