DARPP32 (PPP1R1B) pThr75 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF
Recommend Dilution	Immunofluorescence: 1/100 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthesized KLH phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
Specificity	DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.
Formulation	Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Store at 4°C for short term use. Avoid repeated freezing and thawing.
Predicted Protein Size	32 kd.
Gene Name	protein phosphatase 1 regulatory inhibitor subunit 1B
Database Link	Entrez Gene 19049 MouseEntrez Gene 360616 RatEntrez Gene 84152 Human UniProt ID Q9UD71
Background	DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 and phosphorylated DARPP32 is a potent protein phosphatase 1. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho DARPP32, thereby reducing the phosphatase 1 inhibitory activity of DARPP32.
Synonyms	PPP1R1B, DARPP-32, FLJ20940
Reference Data
Protein Families	Druggable Genome

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