eNOS (NOS3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF
Recommend Dilution	Immunofluorescence: 1/100 - 1/200.
Reactivity	Human Mouse Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of threonine 495(K-K-TP-F-K)
Specificity	eNOS Antibody detects endogenous levels of total eNOS protein.
Formulation	Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	nitric oxide synthase 3
Database Link	Entrez Gene 18127 Mouse UniProt ID P29474
Background	Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
Synonyms	Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway

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