Plakophilin 3 (PKP3) (277-474) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunohiostochemistry on frozen and paraffin sections: 1/100. Western Blot: 1/1000. Immunofluorescence. Positive tissues: - stratified squamous epithelia: palmar, epidermis, scalp skin - stratified epithelia: tongue, vagina, esophagus - transitional epithelia: bladder - non-stratified (single layered) epithelia: colon, duodenum, pancreas, several glands - non-epithelial: reticulum cells of lymph node follicle Negative tissues: - liver, heart, skeletal muscle, endothelia (vessels)
Reactivity	Human
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Human recombinant plakophilin 3 peptide (aa 277-474) purified from E. coli
Specificity	PP3-1 antiserum staining for plakophilin 3 is seen in most desmosomes and partly dispersed in cytoplasm under various fixation conditions (FA, MeAc long, MeAc short):
Formulation	State: Serum State: Liquid serum containing 0.09% sodium azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	plakophilin 3
Database Link	Entrez Gene 11187 Human UniProt ID Q9Y446
Background	Plakophilin 3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
Synonyms	Plakophilin-3
Reference Data

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