MAD3 (MXD3) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC
Recommend Dilution	Peptide ELISA: 1/4000 (Detection Limit).  Western blot: No signal obtained yet but low background observed in NCI-H460, HepG2, Human Liver, Human Heart and Human Brain lysates at up to 3 µg/ml.  Immunocytochemistry: This product has been successfully used by an anonymous Customer on Methanol-fixed and 0.5% TX100-permeabilized HeLa cells, staning the cytoplasm.  Immunohistochemistry: An anonymous Customer found positive results on Human HeLa cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-Terminus of Human MAD3 (NP_112590.1)
Specificity	This antibody is expected to recognise isoform a (NP_112590.1) only.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MAX dimerization protein 3
Database Link	Entrez Gene 83463 Human UniProt ID Q9BW11
Background	MAD3 contains 1 basic helix-loop-helix (bHLH) domain. It is a transcriptional repressor and binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
Synonyms	MAD-3, MXD3,Max-interacting transcriptional repressor MAD3
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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