Bcl2 Binding component 3 (BBC3) (BH3 Domain) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/100-1/500. Flow Cytometry: 1/10-1/50. Immunofluorescence: 1/10-1/50. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide selected from the C-terminal region of Human PUMA
Specificity	This antibody is specific to Human and Mouse PUMA (BH3 Domain). Other species not tested.
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	20532 Da
Gene Name	BCL2 binding component 3
Database Link	Entrez Gene 27113 Human UniProt ID Q96PG8
Background	PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms	BBC3, JFY-1
Reference Data

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