CDC25B Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA 1:1,000. Western blot 1:50 - 1:100. Immunohistochemistry 1:10 - 1:50.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human CDC25B.
Specificity	This antibody detects CDC25B at center.
Formulation	PBS with 0.09% (W/V) sodium azide State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	First purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	cell division cycle 25B
Database Link	Entrez Gene 994 Human UniProt ID P30305
Background	CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.
Synonyms	CDC25HU2
Note	Molecular weight: 64987 Da
Reference Data

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