KIF4A (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, WB
Recommend Dilution	Peptide ELISA: 1/64000 (Detection Limit).   Western blot: 1-2 µg/ml.  Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2). Primary incubation: 1 hour at room temperature. Negative Control: Human Ovary lysate.  Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus of U2OS cells.  Flow Cytometry analysis of HEK293 cell: 10µg/ml.
Reactivity	Bovine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-Terminus of Human KIF4A (NP_036442.3)
Specificity	This antibody detects KIF4A at C-term.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	kinesin family member 4A
Database Link	Entrez Gene 24137 Human UniProt ID O95239
Background	Kinesins, such as KIF4A are required for mitotic chromosomal positioning and bipolar spindle stabilization. It is located in the nucleus and is associated with mitotic chromosomes. It is highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
Synonyms	KIF4A, KIF4, Chromosome-associated kinesin KIF4A, Chromokinesin-A
Reference Data

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